Neuroprotective effect of Petroleum-ether (PE), Ethyl acetate (EA) and Chloroform fractions (CHCl3) from leaves of Ocimum sanctum (OS) were investigated on 6-hydroxy dopamine (6-OHDA) lesioned rodent model of Parkinson disorder (PD). PE, EA and CHCl3 fractions from ethanolic extract of the leaves of OS were pretreated (300 mg/kg) three weeks before unilateral injection of 6-OHDA on 22nd day (10µg/2µl) into the right striatum. The behavioral observations, oxidative biochemical markers, Dopamine D2 receptor binding assay, Quantification of Dopamine (DA) and its metabolites and tyrosine hydroxylase (TH) immunohistochemistry were evaluated after three weeks of leison. Increase in apomorphine-induced rotations and deficits in locomotor activity & muscular coordination due to lesion were significantly restored in EA treated group, PE and CHCl3 fractions leaves of OS. Pretreated animals showed significant protection against neuronal degeneration compared to leison animals by normalizing the deranged levels of biomarkers with EA, PE, and CHCl3 fractions of the leaves of OS. The results were further supported by D2 receptors binding assay, Quantification of DA and its metabolites and TH immunohistochemistry study. Different extract of OS significantly prevented neurodegenration associated with PD in rats. Further, OS annetuated the neuropsychological symptoms associated with animal models of PD. These neuroprotective effects observed with OS can be attributed to its anti-oxidant activity.
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